Associate Professor
manjushaniser.ac.in
+91-674-2494195
Angiogenesis and Tumorigenesis Regulation: Molecular Mechanism and Genetic Predisposition
Academic Publications
Jeeyar V, Prasad Singh S, Dixit M. Functional relevance of MMP2 promoter variants in gallbladder cancer: A case-control study in an Eastern Indian Population. Gene. 2024;913:148372. doi:10.1016/j.gene.2024.148372
Patel SA, Hassan MK, Naik M, Mohapatra N, Balan P, Korrapati PS, Dixit M. EEF1A2 promotes HIF1A mediated breast cancer angiogenesis in normoxia and participates in a positive feedback loop with HIF1A in hypoxia. Br J Cancer. 2024 Feb;130(2):184-200. doi:10.1038/s41416-023-02509-2
Patel SA, Hassan MK, Dixit M. Oncogenic activation of EEF1A2 expression: a journey from a putative to an established oncogene. Cell Mol Biol Lett. 2024;29(1):6. Published 2024 Jan 3. doi:10.1186/s11658-023-00519-9
Subhashree S Panda, Supriya Kumari, Manjusha Dixit, Nagendra K Sharma. N-Salicyl-AAn-picolamide Foldameric Peptides Exhibit Quorum Sensing Inhibition of Pseudomonas aeruginosa (PA14). ACS Omega 2023, 8, 33, 30349–30358. Publication Date: August 8, 2023. https://pubs.acs.org/doi/full/10.1021/acsomega.3c03404
Vinay J., A Palo, K Besra, M Dixit. Gallbladder cancer-associated genetic variants rs1003349 and rs1004030 regulate MMP14 expression by altering SOX10-and MYB-binding sites. Human Molecular Genetics, Volume 32, Issue 14, 15 July 2023, Pages 2357–2372. doi.org/10.1093/hmg/ddad077
B Mukherjee, P Brahma, T Mohapatra, S Chawla, M Dixit. Reduced FRG1 expression promotes angiogenesis via activation of the FGF2â€mediated ERK/AKT pathway. FEBS Open Bio, 2023, 13, 5, 804-817. (https://doi.org/10.1002/2211-5463.13582)
Ananya Palo, Saket Awadhesbhai Patel, Bibekananda Sahoo, Tirumala Kumar Chowdary, Manjusha Dixit. FRG1 is a direct transcriptional regulator of nonsense-mediated mRNA decay genes. Genomics, 2023,115,1,110539.
Dixit, M., J, V., Choudhury, S. (2023). Targeted Therapies in Gallbladder Cancer: Current Status and Future Perspectives. In: Kumar Shukla, V., Pandey, M., Dixit, R. (eds) Gallbladder Cancer. Springer, Singapore. https://doi.org/10.1007/978-981-19-6442-8_16
BB Palai, SA Patel, NK Sharma, M Dixit. One-pot synthesis of cyclic-aminotropiminium carboxylate derivatives with DNA binding and anticancer properties. Communications Chemistry, 2022, 5 (1), 179
T Mohapatra, M Dixit. IQ Motif Containing GTPase Activating Proteins (IQGAPs), A-Kinase Anchoring Proteins (AKAPs) and Kinase Suppressor of Ras Proteins (KSRs) in Scaffolding Oncogenic Pathways and Their Therapeutic Potential. ACS Omega, 2022, 7, 50, 45837–45848. (Invited)
B Mukherjee, A Tiwari, A Palo, N Pattnaik, S Samantara, M Dixit. Reduced expression of FRG1 facilitates breast cancer progression via GM-CSF/MEK-ERK axis by abating FRG1 mediated transcriptional repression of GM-CSF. Cell Death Discovery, 2022, 8 (1), 1-14
S Meher, S Kumari, M Dixit, NK Sharma. Cuâ€Catalyzed Synthesis of Alkylaminotroponyl Sulfones as Pseudomonas Aeruginosa Quorum Sensing Inhibitors Targeting lasI/R QS Circuitry. Chemistry–An Asian Journal, 2022, 17 (23), e202200866. (Co-corresponding author)
B Bhusana Palai, S Kumari, M Dixit, NK Sharma. Nonbenzenoid BODIPY Analogues: Synthesis, Structural Organization, Photophysical Studies, and Cell Internalization of Biocompatible N-Alkyl-Aminotroponyl Difluoroboron. ACS omega, 2022, 7 (31), 27347-27358
D Kumar, SA Patel, R Khan, S Chawla, N Mohapatra, M Dixit. IQ motif-containing GTPase-activating protein 2 inhibits breast cancer angiogenesis by suppressing VEGFR2-AKT signalling. Molecular Cancer Research, 2022, 20 (1): 77–91
KK Sinha, J Vinay, S Parida, SP Singh, M Dixit. Association and Functional Significance of Genetic Variants Present in Regulatory Elements of SERPINB5 Gene in Gallbladder Cancer. Gene, 2022, 188: 1145989
Khan, R., Palo, A. & Dixit, M. Role of FRG1 in predicting the overall survivability in cancers using multivariate based optimal model. Sci Rep, 2021, 11, 22505
D Kumar, SA Patel, MK Hassan, N Mohapatra, N Pattanaik, M Dixit. Reduced IQGAP2 expression promotes EMT and inhibits apoptosis by modulating the MEK-ERK and p38 signaling in breast cancer irrespective of ER status. Cell death & disease, 2021, 12 (4), 1-14
J Vinay, Debakanta Mishra, Dinesh Meher, Sashibhusan Dash, Kusumbati Besra, Niharika Pattnaik, Shivaram Prasad Singh, Manjusha Dixit. Genetic association of MMP14 promoter variants and their functional significance in gallbladder cancer pathogenesis. Journal of Human Genetics, 2021, 1-10
B Mahananda, J Vinay, A Palo, A Singh, SK Sahu, SP Singh, M Dixit. SERPINB5 Genetic Variants rs2289519 and rs2289521 are Significantly Associated with Gallbladder Cancer Risk. DNA and cell biology, 2021, 40 (5), 706-712
A Bollu, MK Hassan, M Dixit, NK Sharma. The 2′-caged-tethered-siRNA shows light-dependent temporal controlled RNAi activity for GFP gene into HEK293T cells. Bioorganic & Medicinal Chemistry, 2021, 30, 115932
MK Hassan, D Kumar, SA Patel, M Dixit. EEF1A2 triggers stronger ERK mediated metastatic program in ER negative breast cancer cells than in ER positive cells. Life Sciences, 2020, 262, 118553
Hassan MK, Kumar D, Patel SA, Pattanaik N, Mohapatra N, Dixit M. Expression pattern of EEF1A2 in brain tumors: Histological analysis and functional role as a promoter of EMT. Life Sci. 2020, Apr 1;246:117399. doi: 10.1016/j.lfs.2020.117399. Epub 2020 Feb 4. PubMed PMID: 32032648.
Tiwari A, Mukherjee B, Hassan MK, Pattanaik N, Jaiswal AM, Dixit M. Reduced FRG1 expression promotes prostate cancer progression and affects prostate cancer cell migration and invasion. BMC Cancer. 2019;19(1):346.
Naik, M.; Brahma, P.; Dixit, M. A Cost-Effective and Efficient Chick Ex-Ovo CAM Assay Protocol to Assess Angiogenesis. Methods Protoc. 2018, 1, 19.
Hassan MK, Kumar D, Naik M, Dixit M. The expression profile and prognostic significance of eukaryotic translation elongation factors in different cancers. PLoS One. 2018 Jan 17;13(1):e0191377. doi: 10.1371/journal.pone.0191377.
Kumar D, Hassan MK, Pattnaik N, Mohapatra N, Dixit M. Reduced expression of IQGAP2 and higher expression of IQGAP3 correlates with poor prognosis in cancers. PLoS One. 2017 Oct 26;12(10): e0186977. doi: 10.1371/journal.pone.0186977.
Tiwari A, Mukherjee B, Dixit M. MicroRNA Key to Angiogenesis Regulation: miRNA Biology and Therapy. Curr Cancer Drug Targets. 2017 Jun 30. doi: 10.2174/1568009617666170630142725. PubMed PMID: 28669338.
Tiwari A, Pattnaik N, Mohanty Jaiswal A, Dixit M. Increased FSHD region gene1 expression reduces in vitro cell migration, invasion, and angiogenesis, ex vivo supported by reduced expression in tumors. Biosci Rep. 2017 Oct 27;37(5). pii: BSR20171062. doi: 10.1042/BSR20171062.
Arman Kunwar Hansda, Ankit Tiwari, Manjusha Dixit. Current Status and Future Prospect of FSHD Region Gene 1. J Biosci. 2017 Jun;42(2):345-353.
Gade CR, Dixit M, Sharma NK. Dideoxy nucleoside triphosphate (ddNTP)analogues: Synthesis and polymerase substrate activities of pyrrolidinyl nucleoside triphosphates (prNTPs). Bioorg Med Chem. 2016 Sep 15;24(18): 4016-22.doi: 10.1016/j.bmc.2016.06.043.
Deepa Kumari, Ankit Tiwari, Mahasweta Choudhury, Abhishek Kumar, Aparna Rao, Manjusha Dixit. A novel KERA mutation in a case of autosomal recessive cornea plana with primary angle closure glaucoma. J Glaucoma. 2016 Feb;25(2):e106-9.
Tiwari A, Gade CR, Dixit M, Sharma NK. Methylene Salicylicacidyl Hexamer (MSH) Has DNAse Activity. Appl Biochem Biotechnol. 2015 Jul;176(6):1791-800.
Subhransu Sekhar Sahoo, Sebaranjan Biswal, and Manjusha Dixit. Distinctive Mutation Spectrum of the HBB Gene in an Urban Eastern Indian Population. Hemoglobin 2014, 38: 33-38.
Sachchida N. Pandey, Jennifer Cabotage, Rongye Shi, Manjusha Dixit, Margret Sutherland, Jian Liu, Stephanie Muger, Scott Q. Harper, Kanneboyina Nagaraju, and Yi-Wen Chen. Conditional over-expression of PITX1 causes skeletal muscle dystrophy in mice. Biology Open 2012, 1:629-639.
Sharma, N.K., P. Lunawat and M. Dixit. Binding studies of natural product berberine with DNA G-quadruplex. Am. J. Biochem. Biotechnol.2011, 7: 130-134.
Yang Z, Tong Z, Chen Y, Zeng J, Lu F, Sun X, Zhao C, Wang K, Davey L, Chen H, London N, Muramatsu D, Salasar F, Carmona R, Kasuga D, Wang X, Bedell M, Dixit M, Zhao P, Yang R, Gibbs D, Liu X, Li Y, Li C, Li Y, Campochiaro B, Constantine R, Zack DJ, Campochiaro P, Fu Y, Li DY, Katsanis N, Zhang K. Genetic and functional dissection of HTRA1 and LOC387715 in age-related macular degeneration. PLoS Genet. 2010 Feb 5;6(2):e1000836 (Name misspelled).
Gibbs D, Yang Z, Constantine R, Ma X, Camp NJ, Yang X, Chen H, Jorgenson A, Hau V, Dewan A, Zeng J, Harmon J, Buehler J, Brand JM, Hoh J, Cameron DJ, Dixit M, Tong Z, Zhang K. Further mapping of 10q26 supports strong association of HTRA1 polymorphisms with age-related macular degeneration. Vision Res. 2008 Feb;48(5):685-689.
Dixit M, Ansseau E, Tassin A, Winokur S, Shi R, Qian H, Sauvage S, Mattéotti C, van Acker AM, Leo O, Figlewicz D, Barro M, Laoudj-Chenivesse D, Belayew A, Coppée F, Chen YW. DUX4, a candidate gene of facioscapulohumeral muscular dystrophy, encodes a transcriptional activator of PITX1. Proc Natl Acad Sci U S A. 2007 Nov 13;104(46):18157-62.
Dixit M, Choudhuri G, Saxena R, Mittal B. Association of apolipoprotein A1-C3 gene cluster polymorphisms with gallstone disease. Can J Gastroenterol. 2007 Sep;21(9):569-75.
Pandey SN, Srivastava A, Dixit M, Choudhuri G, Mittal B. Haplotype analysis of signal peptide (insertion/deletion) and XbaI polymorphisms of the APOB gene in gallbladder cancer. Liver Int. 2007 Sep;27(7):1008-15.
Srivastava A, Pandey SN, Dixit M, Choudhuri G, Mittal B. Cholecystokinin receptor A gene polymorphism in gallstone disease and gallbladder cancer. J Gastroenterol Hepatol. 2008 Jun;23(6):970-5.
Dixit M, Choudhuri G, Mittal B. Association of lipoprotein receptor, receptor-associated protein, and metabolizing enzyme gene polymorphisms with gallstone disease: A case-control study. Hepatol Res. 2006 Sep;36(1):61-9.
Dixit M, Choudhuri G, Keshri LJ, Mittal B. Association of low density lipoprotein receptor related protein-associated protein (LRPAP1) gene insertion/deletion polymorphism with gallstone disease. J Gastroenterol Hepatol. 2006 May;21(5):847-9.
Dixit M, Choudhuri G, Mittal B. Association of APOE-C1 gene cluster polymorphisms with gallstone disease. Dig Liver Dis. 2006 Jun;38(6):397-403.
Pandey SN, Dixit M, Choudhuri G, Mittal B. Lipoprotein Receptor Associated Protein (LRPAP1) Insertion/Deletion Polymorphism: Association with Gallbladder Cancer Susceptibility. Int J Gastrointest Cancer. 2006;37(4):124-8.
Dixit M, Bhattacharya S, Mittal B. Association of CETP TaqI and APOE polymorphisms with type II diabetes mellitus in North Indians: a case control study. BMC Endocr Disord. 2005 Jul 1;5:7.
Dixit, Manjusha; Mittal, B. Frequencies of CETP gene TaqI B and D442G polymorphisms in North Indian Population. Current Science, 2005, 88, 1973-1977.
Agarwal, G. S.; Kamboj, D. V.; Alam, S. I.; Dixit, Manjusha; Singh, L. Environmental detection of bacillus anthracis spores. Current science, 2002 83, 697.
Dr. Dixit's research interest is to understand the genetics and molecular biology of various human genetic diseases, especially cancer.
Abnormal growth of new blood vessels plays an important role in many diseases, including cancer. To treat cancer, various potential anti-angiogenesis drugs have been tested with limited success. Blocking just one regulatory pathway may not be enough. Till date, all these angiogenic-switch regulatory molecules and their mechanism are not known. In order to do that, my research group is interested towards the validation and elucidation of the molecular mechanism of putative angiogenic regulators. We are also interested in finding out the role and molecular mechanism of these newly identified angiogenesis regulators, in tumorigenesis and tumor angiogenesis. We started with FRG1 (a putative angiogenic regulator) and further research led to identification of EEF1A2 and IQGAP2, as its interacting partners. Currently, we have established FRG1 to have reduced expression in cancer and its effect on various properties of cells (proliferation, migration and invasion). We found that IQGAP2 expression is also reduced but the expression of EEF1A2 is increased in tumor tissues. We discovered the mechanism of, how FRG1 and IQGAP2 behave as tumor suppressor genes but EEF1A2 behaves as an oncogene. Our data suggests that all three genes may impart tumorigenic potential by affecting angiogenesis.
Another major area of our research includes understanding molecular mechanism of gallbladder cancer and establishment of genetic risk factors in Indian population. So far, we have
found that SNPs present in MMP14 affect it's expression and might be responsible for altered risk in the population of Odisha.
Dr. Dixit regularly offers two theory and two practical courses in odd or even semeter.
-Member secretary Institution Ethics committee, NISER
